Generator

Part:BBa_I766998:Design

Designed by: Jimmy Jie Min Huang   Group: iGEM07_UCSF   (2007-08-16)


YopJ-RR under inducible promotor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2251
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2251
    Illegal NotI site found at 2810
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1300
    Illegal BamHI site found at 1141
    Illegal BamHI site found at 2793
    Illegal XhoI site found at 1891
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2251
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2251
    Illegal NgoMIV site found at 172
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1069
    Illegal SapI.rc site found at 1093


Design Notes

Device was created using a method of combinatorial cloning using the type IIs restriction endonuclease AarI. Each device contains adaptor regions in between coding regions (RFP, effectors, and zippers) for this method.


Source

Composite Part.

References